Immunohistochemical Evaluation of VDAC2 and SOX2

Immunohistochemical staining was performed using Dako REALTM EnVision^TM Detection System (K5007, Dako, Glostrup, Denmark) in accordance with the manufacturer’s protocol. Rabbit anti-human VDAC2 polyclonal antibody (ab126120, Abcam) and mouse anti-human SOX2 (ab171380, Abcam) were used as the primary antibodies. Immunohistochemical scores of VDAC2 and SOX2 were calculated independently by two histopathologists in a blinded manner according to the staining intensity and percentage of positive tumor cells^{68 (link)}. The best predictive cutoff value of VDAC2 expression was determined to be 6 analyzed by X-tile software. The score ≥ 6 was defined as VDAC2^high, otherwise was defined as VDAC2^low.

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Zhou K., Yao Y.L., He Z.C., Chen C., Zhang X.N., Yang K.D., Liu Y.Q., Liu Q., Fu W.J., Chen Y.P., Niu Q., Ma Q.H., Zhou R., Yao X.H., Zhang X., Cui Y.H., Bian X.W., Shi Y, & Ping Y.F. (2018). VDAC2 interacts with PFKP to regulate glucose metabolism and phenotypic reprogramming of glioma stem cells. Cell Death & Disease, 9(10), 988.

Publication 2018

Dako REALTM EnVisionTM Detection System ab126120 ab171380

Anti antibody Antibodies Human Mouse Rabbit Sox2 Tumor Vdac2

Corresponding Organization :

Other organizations : Army Medical University, Southwest Hospital

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Variable analysis

independent variables

VDAC2 expression (high vs. low)

dependent variables

Immunohistochemical scores of VDAC2 and SOX2

control variables

Staining performed using Dako REALTM EnVision^TM Detection System (K5007, Dako, Glostrup, Denmark) according to manufacturer's protocol
Rabbit anti-human VDAC2 polyclonal antibody (ab126120, Abcam) and mouse anti-human SOX2 (ab171380, Abcam) used as primary antibodies
Immunohistochemical scores calculated independently by two histopathologists in a blinded manner

controls

No positive or negative controls explicitly mentioned

Annotations

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