Metabolites were analyzed on an Agilent 7890/5975C gas chromatography–mass spectrometry system (Agilent Technologies, Santa Clara, CA, USA) using selected ion monitoring methods described in a previous report.9 (link) Peaks were integrated in MSD ChemStation (Agilent Technologies), and correction for natural isotope abundance was performed using IsoCor software.10 (link) Corrected metabolite signals were converted to molar amounts by comparing metabolite peak abundances in samples with those in a “standard mix” containing known quantities of metabolites run along each individual experiment. These known metabolite concentrations were used to generate a standard curve that allowed for metabolite quantification.
Protocol detail
Metabolite Quantification by GC-MS
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : University of Washington
Other organizations : Seattle Children's Hospital
Top 5 similar protocols
Variable analysis
independent variables
- None explicitly mentioned
- Metabolite signals (peak abundances)
- None explicitly mentioned
- Positive control: 'Standard mix' containing known quantities of metabolites, used to generate a standard curve for metabolite quantification.
- Negative control: Not explicitly mentioned.
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!