Thirty-two gauge catheters were inserted through the atlanto-occipital membrane and extended to T8 (36 (link)). The location of each catheter was confirmed following euthanasia. Rats received either BDNF antagonist trkB-Fc (R&D Systems, Minneapolis, MN), 5 µg in 10 µl sterile saline or vehicle once/day during the 9-day stress period or BDNF siRNA or control siRNA (2 µg of the appropriate siRNA; 1:5 v/v with i-Fect transfection reagent Neuromics, Edina, MN). Each rat received 2ug siRNA/10 µl.) on days 1, 3, 5 and 7 of the 9 day stress period.
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Intraspinal Catheter Implantation and BDNF Modulation
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Corresponding Organization : The University of Texas Medical Branch at Galveston
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Variable analysis
independent variables
- BDNF antagonist trkB-Fc
- BDNF siRNA
- Control siRNA
- Location of each catheter confirmed following euthanasia
- Thirty-two gauge catheters inserted through the atlanto-occipital membrane and extended to T8
- Rats received BDNF antagonist trkB-Fc or vehicle once/day during the 9-day stress period
- Rats received BDNF siRNA or control siRNA (2 µg of the appropriate siRNA; 1:5 v/v with i-Fect transfection reagent) on days 1, 3, 5 and 7 of the 9 day stress period
- Positive control: BDNF antagonist trkB-Fc
- Negative control: Vehicle
- Negative control: Control siRNA
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