HPLC Analysis of Ferulate Transesterification

Analysis of ferulates (donor and products) was performed by HPLC using a 100–5 C18 Nucleosil column (250 mm × 4.6 xx) (Macherey Nagel, Düren, Germany) at isocratic conditions (70:30 v/v acetonitrile: water), 0.6 mL min⁻¹ and room temperature while detection was done by a PerkinElmer Flexar UV/Vis detector at 300 nm as described previously [11 (link),12 ]. The yield was defined as the molar amount of generated transesterification product compared to the initial amount of limiting reactant, expressed as percentage. The overall conversion of VFA was defined as the sum of molar amount of products (PFA or AFA and FA) compared to the initial amount of donor, expressed as percentage. The rate was expressed as the molar concentration of generated transesterification product per hour per amount of enzyme. The selectivity was defined as the molar concentration of generated transesterification product divided by the concentration of generated FA.