Telemetric Bladder Function Assessment

Mice were anaesthetised with isoflurane (1–5%), and an HDX11 telemeter (Data Sciences International Inc., New Brighton, MN, USA; Figure 2A) implanted subcutaneously via a laparotomy with the pressure line inserted into the bladder and secured by a suture. Animals recovered over 7–10 days with prophylactic antibiotics and analgesics. After recovery, animals were housed in modified metabolic cages where telemeter recordings were obtained through receiving units (RSC-1, Data Sciences International Inc.). Load cells (5 μl/10 Hz sensitivity) below each cage measured the duration and volume of voids, and filter paper recorded voiding patterns. Data were recorded with 12-hour light/dark cycles using LabChart (ADInstruments, Colorado Springs, CO, USA) and OxyMax (Columbus Instruments, Columbus, OH, USA) software. Arterial blood pressure (BP) and heart rate (HR) were measured using a BP-2000 analysis system (Visitech Systems, Apex, NC, USA) before cinaciguat administration and then immediately, 1 h and 2 h after administration. Animals were acclimatised to the procedure for a week before cinaciguat treatment. Decerebrate CMG recordings were conducted as previously described [27 (link)].

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Zabbarova I.V., Ikeda Y., Kozlowski M.G., Tyagi P., Birder L., Chakrabarty B., Perera S., Dhir R., Straub A.C., Sandner P., Andersson K.E., Drake M., Fry C.H, & Kanai A. (2022). Benign prostatic hyperplasia/obstruction ameliorated using a soluble guanylate cyclase activator. The Journal of pathology, 256(4), 442-454.

Publication 2021

LabChart OxyMax BP-2000 analysis system

Analgesics Animals Antibiotics Bladder Blood pressure Cells Cinaciguat Heart rate Isoflurane Laparotomy Mice Paper Pressure Prophylactic Sensitivity Springs Suture Telemeter

Corresponding Organization : University of Pittsburgh

Other organizations : University of Bristol, Bayer (Germany), Lund University

Top 5 similar protocols

Variable analysis

independent variables

Administration of cinaciguat

dependent variables

Arterial blood pressure (BP)
Heart rate (HR)
Voiding patterns
Duration and volume of voids

control variables

Isoflurane anesthesia
HDX11 telemeter implantation
7-10 days recovery period with prophylactic antibiotics and analgesics
Housing in modified metabolic cages
12-hour light/dark cycles
Acclimatization to the procedure for a week before cinaciguat treatment

positive controls

Decerebrate CMG recordings as previously described [27]

negative controls

Not explicitly mentioned

Annotations

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