RNA was treated to deplete rRNA using Ribo-Minus technology. Libraries were prepared from purified RNA using ScriptSeq™ v2 and were sequenced on an Illumina HiSeq platform. We obtained 60 million paired-end reads of 50 bp length. Read mapping was done with STAR aligner using default settings with the option –outSAMtype BAM SortedByCoordinate (22 (link)) with default settings. For known transcript models we used GRCm38.100 Ensembl annotations downloaded from Ensembl repository (23 (link)). Counting reads over gene model was carried out using GenomicFeatures Bioconductor package (24 (link)). The aligned reads were analysed with custom R scripts in order to obtain gene expression measures. For normalization of read counts and identification of differentially expressed genes we used DESeq2 with Padj < 0.01 cutoff (25 (link)). GO term and KEGG pathways were analysed using g:Profiler (26 (link)) and metascape (27 (link)). The data are deposited to GEO and can be downloaded under the accession number GSE186703.