Western Blot Analysis of LXRα, TNF-α, IFN-γ, and ABCA1

Total protein extracts were prepared as described [17 (link)]. Equal amounts of protein were resolved by SDS-PAGE and analyzed with anti-LXRα (1 : 1,000, ab135039, Abcam, Cambridge, MA), anti-TNF-α (1 : 1,000, ab183896, Abcam), anti-interferon-γ (IFN-γ; 1 : 1,000, EPR1108, Abcam), anti-ABCA1 (PA1-16789, Thermo scientific, Fremont, CA), and anticytoskeletal actin (1 : 10,000, A300-491A, Bethyl Laboratories, Montgomery, TX) antibodies. The secondary antibody used with each was goat anti-mouse antibody (1 : 2,000, AbFrontier, Seoul, Korea), except for anticytoskeletal actin (1 : 20,000). Following transfer and blotting, the proteins of interest were visualized by enhanced chemiluminescence (Pierce, Rockford, IL) and analyzed.