Molecular Cloning and Genetic Manipulation Protocols

Bacteria used in this study (Table S1) were cultured in TY (Beringer, 1974 ) or UMS (Brown & Dilworth, 1975 ; Haskett, Paramasivan, et al., 2022b ; Poole et al., 1994 (link)). Plasmids (Table S2 and Data S2) were constructed using HiFi assembly (New England Biolabs) or BEVA modular golden‐gate assembly (Geddes, Mendoza‐Suárez, & Poole, 2019a (link); Weber et al., 2011 (link)) and were mobilized into strains of interest via di‐parental mating with E. coli ST18 (Thoma & Schobert, 2009 (link)). For mini‐Tn7 integration into the chromosome of AcLP, tri‐parental matings were required to additionally mobilize the transposase helper plasmid pTNS3 (Choi & Schweizer, 2006 (link)). 5′‐RACE was performed using a 2nd Generation Roche 5′/3′ RACE Kit as per the manufacturer's recommendations.