Cell staining using an actin cytoskeleton and focal adhesion staining kit (Catalog Number FAK100, Millipore, Burlington, MA, USA) was performed after 2, 7, and 14 days of culture following the instructions provided by the manufacturer [38 (link)]. Microscopy was performed with a fluorescent microscope (Revolve4 RVL-100-G, Echo, San Diego, CA, USA).
Free full text: Click here