Flow Cytometry Analysis of Bone Marrow and Spleen Cells

Bone marrow and spleen cells were stained for flow cytometry as previously described (Mayer et al., 2017 (link)). Gating strategy for BM erythroid cells was described in the Supplementary Figure 1. Cells were stained with the following antibodies: CD45-FITC (30-F11, eBioscience), CD11b-FITC (M1/70, eBioscience), Gr-1-FITC (RB6-8C5, eBioscience), Ter119-APC (TER119, Biolegend) and CD44-PE (IM7, Biolegend), F4/80-APC (BM8, Biolegend), Gr-1-FITC (RB6-8C5, Biolegend), CD3-PE (145-2C11, Biolegend), Ly6C-PE (HK1.4, Biolegend), CD106-PE (429, Biolegend), CD45R/B220-APC (RA3.682, Biolegend). Cells were analyzed using AccuriC6 flow cytometer and data were analyzed using FlowJo software (v10.7.1).

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Myneni V.D., Szalayova I, & Mezey E. (2021). Differences in Steady-State Erythropoiesis in Different Mouse Bones and Postnatal Spleen. Frontiers in Cell and Developmental Biology, 9, 646646.

Publication 2021

CD45-FITC (30-F11, CD11b-FITC (M1/70, Gr-1-FITC (RB6-8C5, CD44-PE (IM7, F4/80-APC (BM8, CD3-PE (145-2C11, Ly6C-PE (HK1.4, CD106-PE

Antibodies Bone marrow Cd11b Cd44 Cells Erythroid cells Fitc Flow cytometry Spleen

Corresponding Organization : National Institutes of Health

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Erythroid cells in bone marrow and spleen

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