The Rigol L3000 HPLC system with a C18 column (Waters BEH C18, 4.6×250 mm, 5 μm) was used to fractionate the separation as the method and conditions described by the previous study [12 (link)].
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HPLC Separation Protocol Using Rigol L3000 and C18 Column
Corresponding Organization : Jilin Agricultural University
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Variable analysis
independent variables
- Type of HPLC system (Rigol L3000)
- Type of column (Waters BEH C18, 4.6x250 mm, 5 μm)
- Fractionation of the separation
- Method and conditions described in the previous study [12]
- Positive control: Not specified
- Negative control: Not specified
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