Tissue immunostaining for XIAP, in FFPE sections of normal biliary ducts and CCAs was performed as previously described [20 (link)]. XIAP (#2945, Lifespan Biosciences, Inc.) antibody was diluted in TBS-T-goat serum and incubated overnight at 4 °C. Sections were stained with DAB Peroxidase Substrate Kit and counterstained with hematoxylin QS. Images were captured with a Nikon 90i Upright Microscope equipped with a Nikon Digital camera.
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