The following plasmids were used: pFLAG-Liprin-α1 and pEGFP-Liprin-α1 (human liprin-α1) and FLAG-βGal36 (link); pEGFP-C1, m-Cherry-C1, and pRFP (Clontech Laboratories, MountainView, CA); pEGFP-ERC1a (murine ERC1a) from Y. Takai29 (link); pEGFP-LL5β (murine LL5β) from J. Sanes13 (link); pEGFP-LifeAct37 (link); mCherry-ERC1a5 (link). RFP-LifeAct was obtained from pEGFP-LifeAct by cloning into pRFP vector. GFP-paxillin was a kind gift from dr. Victor Small (Austrian Academy of Sciences, Vienna, Austria). The plasmids pSGT-Y527F-Src38 (link) and RFP-cortactin39 (link) were as described.
SiRNAs were from Qiagen (Hilden, Germany) and Life Technologies. SiRNAs for human proteins were as published4 (link),5 (link),9 (link),34 (link), and targeted the following sequences: liprin-α1 CCAAGGTACAAACTCTTAA; ERC1a: GTGGGAAAACCCTTTCAAT and CCAACAGTACGGGAGGGAG; LL5β: GGAGATTTTGGATCATCTA and GGATCTACCTCATAGCGTA. LL5α: CCATCAGCCTGAGTGAATA; control siRNA for luciferase: CATCACGTACGCGGAATAC. SiRNAs for mouse proteins targeted the following sequences: liprin-α1: GCTGGATGCTATCAACAAA and ERC1a: GAAGGAAGTATTAAGAGAA were as described40 (link); LL5β: AGAGAAGAACAATCTAATA.
SiRNAs were from Qiagen (Hilden, Germany) and Life Technologies. SiRNAs for human proteins were as published4 (link),5 (link),9 (link),34 (link), and targeted the following sequences: liprin-α1 CCAAGGTACAAACTCTTAA; ERC1a: GTGGGAAAACCCTTTCAAT and CCAACAGTACGGGAGGGAG; LL5β: GGAGATTTTGGATCATCTA and GGATCTACCTCATAGCGTA. LL5α: CCATCAGCCTGAGTGAATA; control siRNA for luciferase: CATCACGTACGCGGAATAC. SiRNAs for mouse proteins targeted the following sequences: liprin-α1: GCTGGATGCTATCAACAAA and ERC1a: GAAGGAAGTATTAAGAGAA were as described40 (link); LL5β: AGAGAAGAACAATCTAATA.
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