Auxin plates were prepared by adding auxin indole‐3‐acetic acid (Sigma‐Aldrich) from a 400 mM stock solution in ethanol into NGM at the final concentration of 1 mM (Zhang et al., 2015 (link)). For control ethanol plates, the same volume of ethanol was added to NGM. Animals were transferred on auxin or ethanol plates at the L4 stage except for dauer tests for which eggs were laid and grown on auxin or ethanol plates.
For all aging cohorts, 20 μM 5‐fluorouracil (5‐FU, Sigma‐Aldrich) was added to prevent progeny growth. Animals were transferred weekly to fresh plates, without 5‐FU after 2 weeks. The day of transition to L4 is counted as day 0 of adulthood for the cohort.
For all aging cohorts, 20 μM 5‐fluorouracil (5‐FU, Sigma‐Aldrich) was added to prevent progeny growth. Animals were transferred weekly to fresh plates, without 5‐FU after 2 weeks. The day of transition to L4 is counted as day 0 of adulthood for the cohort.
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