Investigating HSV-1 Impact on Rap1b and Cytoskeleton

To confirm the correlation between the virus, Rap1b, and/or the cytoskeleton, or their relationship after treatments with chemicals or plasmids in HSV-1-infected cells, target-protein-specific primary antibodies, fluorescent-labeled secondary antibodies, or a fluorescently labeled chemical (phalloidin) were applied in IFA as previously described [26 (link),31 (link)]. Cell climbing sheets and cell plates were observed with a Leica SP8 confocal system (Leica Microsystems, Wetzlar, Germany). All images were captured and processed using Leica Application Suite X (Leica Microsystems).

Free full text: Click here

Zhang B., Ding J, & Ma Z. (2023). ICP4-Associated Activation of Rap1b Facilitates Herpes Simplex Virus Type I (HSV-1) Infection in Human Corneal Epithelial Cells. Viruses, 15(7), 1457.

Publication 2023

Leica SP8 confocal system Leica Application Suite X

After treatments Antibodies Cell Cytoskeleton Fluorescent antibodies Hsv 1 Phalloidin Plasmids Protein Virus

Corresponding Organization : Xinjiang University

Top 5 similar protocols

Variable analysis

independent variables

Treatments with chemicals or plasmids

dependent variables

Correlation between the virus, Rap1b, and/or the cytoskeleton

control variables

Target-protein-specific primary antibodies
Fluorescent-labeled secondary antibodies
Fluorescently labeled chemical (phalloidin)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!