Plates were imaged using an INCell 1000 automated microscope (GE Healthcare). Images were taken in nine randomly distributed fields in each well of a 96-well plate. The position of each field remained constant in between wells. Image stacks were analysed by an in-house written protocol using the INCell Developer software (GE Healthcare) (Uusimaa et al., 2014 (link); Diot et al., 2015 (link)).
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