Mass spectra were recorded by a Thermo Orbitrap Elite (Thermo Fisher Scientific; Waltham, MA). The instrument settings were as follows: spray voltage, 3.7 kV; sheath gas flow rate, 25 (arbitrary units); and capillary temperature, 275 °C. MS spectra were acquired with 60000 resolution, which has been shown to yield accurate measurements of hydrogen and deuterium composition.64 From mass spectra obtained during HDX-MS experiments, the centroid of each deuterated peptide envelope and the relative deuterium uptake by each peptide were calculated by HDX Workbench.65 (link)
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Assessing Deuterium Uptake by HDX-MS
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Corresponding Organization : National Institute of Standards and Technology
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- Mass spectra were recorded by a Thermo Orbitrap Elite (Thermo Fisher Scientific; Waltham, MA)
- Spray voltage (3.7 kV)
- Sheath gas flow rate (25 arbitrary units)
- Capillary temperature (275 °C)
- Resolution (60000)
- Centroid of each deuterated peptide envelope
- Relative deuterium uptake by each peptide
- Instrument settings were kept constant to ensure valid results
- No positive or negative controls were explicitly mentioned in the provided information.
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