Cytotoxicity Assay of AGS Cells

The cytotoxicity of AGS cells were assessed by measurement of lactate dehydrogenase (LDH) expression in the cultured supernatants using Cytotoxicity Assay Kit (Beyotime, Wuhan, Hubei Province, China). The low level control was defined as LDH expression in the supernatant of AGS cells, while the high level control was defined as LDH expression in the supernatant of Triton X-100 treated AGS cells. The percentage of target cell death was calculated using the following equation: (experiment value − low level control)/(high level control − low level control) × 100% [17 (link)–20 (link)].

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Xu J., Guo R., Jia J., He Y, & He S. (2021). Activation of Toll-like receptor 2 enhances peripheral and tumor-infiltrating CD8+ T cell cytotoxicity in patients with gastric cancer. BMC Immunology, 22, 67.

Publication 2021

Cytotoxicity Assay Kit

Assay Cell death Cells Cytotoxicity Lactate dehydrogenase Triton x 100

Corresponding Organization :

Other organizations : First Affiliated Hospital of Xi'an Jiaotong University, Wuhan No.1 Hospital

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Variable analysis

independent variables

Treatment with Triton X-100

dependent variables

Lactate dehydrogenase (LDH) expression in the cultured supernatants

control variables

Untreated AGS cells

controls

Triton X-100 treated AGS cells
Untreated AGS cells

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