Genetically Engineered Mouse Models for Medulloblastoma Research

Survivin^fl/fl (31 (link)), Ptc^fl/fl^{(65 (link))}, Math1-Cre-ER(66 (link))' Ptch +/-(67 (link)), and Math1CreER;ptc^fl/fl^{(30 (link))} (MERP) mice have been described previously. P4 MERP pups were gavaged with 0.8g/40μl of tamoxifen (T-5648, Sigma, St. Louis, MO) in corn oil to generate tumors. Tumors from Ptch^+/- and MERP mice were used for experiments. To allow for deletion of survivin in tumor cells, Survivin^fl/fl mice were crossed with Ptch^+/- mice to generate the Survivin ^fl/fl; Ptch^+/- (SP) line. CD-1 Nu/Nu mice were from Charles River Laboratories (Wilmington, MA). P7 wild type C57BL/6 pups were obtained from the SBMRI Animal Facility. All mice were maintained in the Animal Facility, and experiments were performed in accordance with national guidelines and regulations, and with the approval of the SBMRI Institutional Animal Care and Use Committee.

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Brun S.N., Markant S.L., Esparza L.A., Garcia G., Terry D., Huang J.M., Pavlyukov M.S., Li X.N., Grant G.A., Crawford J.R., Levy M.L., Conway E.M., Smith L.H., Nakano I., Berezov A., Greene M.I., Wang Q, & Wechsler-Reya R.J. (2014). Survivin as a therapeutic target in Sonic hedgehog-driven medulloblastoma. Oncogene, 34(29), 3770-3779.

Publication 2014

tamoxifen CD-1 Nu/Nu mice

Animal Cells Corn oil Deletion Institutional animal care and use committee Mice Nu nu Ptch River Survivin Tamoxifen Tumor

Corresponding Organization :

Other organizations : Sanford Burnham Prebys Medical Discovery Institute, National Cancer Institute, Cedars-Sinai Medical Center, The Ohio State University, Neurological Surgery, Children's Cancer Center, Baylor College of Medicine, Lucile Packard Children's Hospital, Stanford University, University of California, San Diego, Rady Children's Hospital-San Diego, University of British Columbia, University of Pennsylvania, Duke University

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Variable analysis

independent variables

Tamoxifen treatment in P4 MERP pups

dependent variables

Tumor growth/development

control variables

Survivin^fl/fl mice
Ptch^+/- mice
CD-1 Nu/Nu mice
P7 wild type C57BL/6 pups

controls

Ptch^+/- mice and MERP mice as positive controls
Survivin^fl/fl; Ptch^+/- (SP) line as experimental group

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