Protocol detail

CRISPR Genome Editing Workflow

Find Similar Protocols

For this study the following software and web tools were used: CRISPR Design software, developed by Zhang at the MIT Laboratory in 2015^{26 (link)}, and CRISPR design tool from SYNTHEGO, for the design of sgRNAs; Primer-BLAST (NCBI), for the design of primers to be used in PCR reactions; Verify Guide Design (SYNTHEGO) for the identification of off-target sites of G3 and G8 sgRNAs; Galaxy platform^{31 (link)} and Integrative Genomics Viewer (IGV) tool^{32 (link)} for on-target NGS analysis; GraphPad Prism 7.00, for statistical analysis.

Free full text: Click here

Pappadà M., Bonuccelli O., Buratto M., Fontana R., Sicurella M., Caproni A., Fuselli S., Benazzo A., Bertorelli R., De Sanctis V., Cavallerio P., Simioni V., Tugnoli V., Salvatori F, & Marconi P. (2022). Suppressing gain-of-function proteins via CRISPR/Cas9 system in SCA1 cells. Scientific Reports, 12, 20285.

Publication 2022

CRISPR design tool GraphPad Prism 7.00

Crispr Primers Prism

Top 5 similar protocols

Variable analysis

independent variables

CRISPR Design software, developed by Zhang at the MIT Laboratory in 2015
CRISPR design tool from SYNTHEGO

dependent variables

Design of sgRNAs
Identification of off-target sites of G3 and G8 sgRNAs
On-target NGS analysis

control variables

Primer-BLAST (NCBI), for the design of primers to be used in PCR reactions
Galaxy platform and Integrative Genomics Viewer (IGV) tool for on-target NGS analysis
GraphPad Prism 7.00, for statistical analysis

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!