Bacterial Identification via Biochemical Tests

The samples were inoculated on MacConkey (Becton, Dickson and Company Sparks, MD, USA) and Eosin Methylene Blue (Becton, Dickson and Company, Sparks, MD, USA) agar plates, and both were incubated for 18 h at 37 ± 2 °C. Subsequently, biochemical identification was confirmed by tests of indole, mobility, sulfhydric acid production, glucose fermentation, lactose fermentation, gas production, Simmons citrate, methyl red, Voges-Proskauer, lysine decarboxylase, and urea [41 (link)].

Free full text: Click here

Enciso-Martínez Y., Barrios-Villa E., Sepúlveda-Moreno C.O., Ballesteros-Monrreal M.G., Valencia-Rivera D.E., González-Aguilar G.A., Martínez-Téllez M.A, & Ayala-Zavala J.F. (2022). Prevalence of Antibiotic-Resistant E. coli Strains in a Local Farm and Packing Facilities of Honeydew Melon in Hermosillo, Sonora, Mexico. Antibiotics, 11(12), 1789.

Publication 2022

MacConkey

Acid Agar Citrate Eosin Fermentation Glucose Indole Lactose Lysine decarboxylase Methylene blue Mobility Urea

Corresponding Organization : Centro de Investigación en Alimentación y Desarrollo

Other organizations : Universidad de Sonora

Top 5 similar protocols

Variable analysis

independent variables

Inoculation of samples on MacConkey agar plates
Inoculation of samples on Eosin Methylene Blue agar plates

dependent variables

Biochemical identification confirmed by tests of indole, mobility, sulfhydric acid production, glucose fermentation, lactose fermentation, gas production, Simmons citrate, methyl red, Voges-Proskauer, lysine decarboxylase, and urea

control variables

Incubation of both agar plates for 18 h at 37 ± 2 °C

controls

No positive or negative controls were explicitly mentioned in the information provided.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!