Isolation and Characterization of Mouse Islets and Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells

Mouse islets were isolated from heterozygous human islet amyloid polypeptide (hIAPP) transgenic (hIAPP^+/-) 8-12-week-old FVB/N mice (Jackson Laboratory, Bar Harbor, ME, USA). The Institutional Animal Care and Use Committee of Samsung Biomedical Research Institute approved all animal experimental protocols in this study. Human umbilical cord blood- (hUCB-) MSCs were isolated according to a reported method [23 (link)]. Umbilical cord blood (UCB) units obtained from full-term deliveries were collected from the unborn placenta with the informed consent of the mothers. The human UCB-MSC isolation procedure was approved by the Institutional Review Board of Samsung Medical Center (IRB No. SMC 2019-11-026), and all participants provided informed consent for the use of the umbilical cord in this experimental study. The human monocyte cell line THP-1 was purchased from the Korean Cell Bank (Seoul, Korea) and maintained in complete RPMI 1640 media.

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Lee E., Ha S., Kim G., Kim J.H, & Jin S.M. (2023). Extracellular Vesicles Derived from Three-Dimensional-Cultured Human Umbilical Cord Blood Mesenchymal Stem Cells Prevent Inflammation and Dedifferentiation in Pancreatic Islets. Stem Cells International, 2023, 5475212.

Publication 2023

FVB/N mice

Cell Deliveries Heterozygous Human Institutional animal care and use committee Islet amyloid polypeptide Isolation Korean Line 1 Mice Monocyte Mothers Placenta Transgenic Umbilical cord Umbilical cord blood

Corresponding Organization : Samsung (South Korea)

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Variable analysis

independent variables

Heterozygous human islet amyloid polypeptide (hIAPP) transgenic (hIAPP+/-) mice

dependent variables

Not explicitly mentioned

control variables

8-12-week-old FVB/N mice
Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs)
Human monocyte cell line THP-1

controls

Positive control: Not specified
Negative control: Not specified

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