The olfactometer used to deliver odours under the two-photon microscope was the same one used in the EAG experiment. During an imaging session, the odorants of interest (Geosmin 10–6, Geosmin 10–3, and 10–1 IAA) were presented to the bee in a sequence either as a single odour or as mixtures, and the sequence was repeated 10 times. Each stimulus pulse lasted 3 s with a 12 s inter-stimulus interval and an exhaust system quickly removes the odours from the experimental area. For comparison of response strength and width also 3 floral odours were tested with the same sequences (1-nonanol 5·10–3, acetophenone 5·10–3, and 3-hexanol 5·10–3).
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