Histomorphometric Analysis of Tibial Growth Plates

Stained transverse sections of tibiae were viewed by an eclipse E400 Nikon light microscopy with ×4, ×10, ×20 or ×40 objectives, using light filters. Images were taken by a high -resolution camera (Olympus DP 71) controlled by Cell A software (Olympus, Japan). The thickness of the entire GPs, the proliferative zone (PZ) and the hypertrophic zone (HZ) as well as the number of cells were measured using the Cell A software with a measuring tool feature. Measurements were performed on these sections from 5 different animals from each group. In each slide, 10 random locations throughout the GPs were selected and measured [28 (link),29 (link)].

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Sides R., Griess-Fishheimer S., Zaretsky J., Shitrit A., Kalev-Altman R., Rozner R., Beresh O., Dumont M., Penn S., Shahar R, & Monsonego-Ornan E. (2021). The Use of Mushrooms and Spirulina Algae as Supplements to Prevent Growth Inhibition in a Pre-Clinical Model for an Unbalanced Diet. Nutrients, 13(12), 4316.

Publication 2021

eclipse E400 Nikon light microscopy Cell A software

Animals Cell Hypertrophic Light Light microscopy Tibiae

Corresponding Organization : Hebrew University of Jerusalem

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Variable analysis

independent variables

Magnification of the light microscopy (×4, ×10, ×20 or ×40 objectives)

dependent variables

Thickness of the entire growth plates (GPs)
Thickness of the proliferative zone (PZ)
Thickness of the hypertrophic zone (HZ)
Number of cells in the GPs

control variables

Stained transverse sections of tibiae
Eclipse E400 Nikon light microscopy
High-resolution camera (Olympus DP 71)
Cell A software (Olympus, Japan)
Measuring tool feature in Cell A software
Measurements performed on sections from 5 different animals from each group
10 random locations throughout the GPs selected and measured on each slide

controls

The authors did not specify any positive or negative controls in the provided information.

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