The laboratory culture of the asexual planarian species Girardia tigrina (Turbellaria, Tricladida) was used as a model organism for the experiments. Planarians were kept in a darkened room at a temperature of 20–23 °C. Feeding was performed once a week by larvae of chironomids (Katrinex, Warsaw, Poland). In the experiments, planarians with a body length of 9–11 mm were used. Previously, they were kept without feeding for 7 days.
The effect of two steroid hormones of the glucocorticoid group on the reparative regeneration process was studied: hydrocortisone and a synthetic hormone of this group—methylprednisolone, a well-known agonist of glucocorticoid receptors and to a lesser extent of mineralocorticoids [18 (link)].
The hydrocortisone stock (Sigma, H0888, Saint Louis, MO, USA) in DMSO (Sigma, Saint Louis, MO, USA) at a concentration of 10−1 M or methylprednisolone (Supelco, PHR1717, Bellefonte, PA, USA) in DMSO in concentration of 10−1 M was added into a 50 mL glass with planarians in an amount sufficient to achieve the needed concentration.
The control and experimental group were kept in 50 mL of a solution of tap and distilled water (in a 2:1 ratio). In each glass, 30 individuals were kept for each experimental and control group. The test compound solutions were added to the experimental groups, to achieve the needed concentrations. The experiments were conducted in three independent series.
The effect of two steroid hormones of the glucocorticoid group on the reparative regeneration process was studied: hydrocortisone and a synthetic hormone of this group—methylprednisolone, a well-known agonist of glucocorticoid receptors and to a lesser extent of mineralocorticoids [18 (link)].
The hydrocortisone stock (Sigma, H0888, Saint Louis, MO, USA) in DMSO (Sigma, Saint Louis, MO, USA) at a concentration of 10−1 M or methylprednisolone (Supelco, PHR1717, Bellefonte, PA, USA) in DMSO in concentration of 10−1 M was added into a 50 mL glass with planarians in an amount sufficient to achieve the needed concentration.
The control and experimental group were kept in 50 mL of a solution of tap and distilled water (in a 2:1 ratio). In each glass, 30 individuals were kept for each experimental and control group. The test compound solutions were added to the experimental groups, to achieve the needed concentrations. The experiments were conducted in three independent series.
Free full text: Click here
