Stratification of CD Subjects by NOD2 and ATG16L1 Variants

Genotyping was at Cedars-Sinai Medical Center using the Illumina Immuno-BeadChip array as previously described (2 (link), 19 (link)). All human subjects were homozygous non-risk for the 3 common CD-associated NOD2 variants (tagged by rs2066842-GG, rs2066844-GG, rs2066845-CC. rs5743293-DD) and then stratified by ATG16L1 CD-associated T300A variant status dividing the study subjects into those that were either homozygous non-risk or homozygous risk (as defined by rs2241880). Written informed consent was received from participants prior to inclusion in the study. All study subjects were identified by number and not by name, had not used antibiotics for at least 6 months. Stool, blood samples, and genetic data were obtained from Material and Information Resources for Inflammatory And Digestive Diseases (MIRIAD) IBD Biobank and approved under IRB #3358 and #Pro00027495.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Zhang H., Zheng L., McGovern D.P., Hamill A.M., Ichikawa R., Kanazawa Y., Luu J., Kumagai K., Cilluffo M., Fukata M., Targan S.R., Underhill D.M., Zhang X, & Shih D.Q. (2017). Myeloid ATG16L1 facilitates host-bacteria interactions in maintaining intestinal homeostasis. Journal of immunology (Baltimore, Md. : 1950), 198(5), 2133-2146.

Publication 2017

Immuno-BeadChip array

Antibiotics Blood Digestive diseases Homozygous Inflammatory Stool

Corresponding Organization : Second Hospital of Hebei Medical University

Other organizations : University of California, Los Angeles

Top 5 similar protocols

Variable analysis

independent variables

ATG16L1 CD-associated T300A variant status
Homozygous non-risk or homozygous risk for the 3 common CD-associated NOD2 variants (tagged by rs2066842-GG, rs2066844-GG, rs2066845-CC. rs5743293-DD)

dependent variables

Not explicitly mentioned

control variables

All human subjects were homozygous non-risk for the 3 common CD-associated NOD2 variants (tagged by rs2066842-GG, rs2066844-GG, rs2066845-CC. rs5743293-DD)
All study subjects had not used antibiotics for at least 6 months
Stool, blood samples, and genetic data were obtained from Material and Information Resources for Inflammatory And Digestive Diseases (MIRIAD) IBD Biobank

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!