Laser-Capture Microdissection of Brain Tissue

After fixation in formalin, FFPE brain tissue blocks containing either the superior frontal gyrus of the frontal cortex or hippocampus (level of LGN) were sectioned at 8 µm and collected onto LCM compatible PET slides (Leica). Sections were stained with cresyl violet to localize regions of interest for LCM [15 (link)] and air dried overnight in a loosely closed container. LCM was used to individually microdissect 10 mm² from the frontal cortex (gray matter layers I–IV) and hippocampal CA1-3, and 4 mm² from the hippocampal DG into LC–MS grade water (Thermo Scientific). Microdissected samples were centrifuged for 2 min at 14,000 g and stored at – 80 °C. LCM was performed at 5X magnification with a LMD6500 microscope equipped with a UV laser (Leica).