ECT Protocol in Mice Model

Adult male, 6~8-week old C57BL/6 mice were used (Charles River) and housed in a standard facility. Electroconvulsive treatment (ECT) was achieved with pulses consisted of 1.0 s, 100 Hz, 16–18 mA stimulus of 0.3 ms delivered using the Ugo Basile ECT unit (Model 57800) as previously described^{34 (link)}. Mock mice were handled in parallel without the electrical current delivery.

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Shi H., Zhang X., Weng Y.L., Lu Z., Liu Y., Lu Z., Li J., Hao P., Zhang Y., Zhang F., Wu Y., Delgado J.Y., Su Y., Patel M.J., Cao X., Shen B., Huang X., Ming G.L., Zhuang X., Song H., He C, & Zhou T. (2018). m6A facilitates hippocampus-dependent learning and memory through Ythdf1. Nature, 563(7730), 249-253.

Publication 2018

C57BL/6 mice ECT unit (Model 57800)

Adult C57bl mice Delivery Electrical Electroconvulsive treatment Male Mice Pulses River

Corresponding Organization :

Other organizations : University of Chicago, ShanghaiTech University, University of Pennsylvania, Tongji University, East China Normal University, Nanjing Medical University, Howard Hughes Medical Institute

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Variable analysis

independent variables

Electroconvulsive treatment (ECT) conditions: 1.0 s, 100 Hz, 16–18 mA stimulus of 0.3 ms

dependent variables

Unspecified

control variables

Adult male, 6~8-week old C57BL/6 mice (Charles River)
Housed in a standard facility
Mock mice handled in parallel without the electrical current delivery

controls

Positive control: Electroconvulsive treatment (ECT) group
Negative control: Mock mice group

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