Human primary skin fibroblasts were isolated as described previously (Szebeni et al., 2018 (link)). Briefly, healthy volunteers (age 18–60 years) were enrolled into the study. The punch biopsies were taken from healthy subjects from the breast area undergoing plastic surgery. Primary fibroblasts were obtained from the skin by enzymatic digestion according to a standard protocol. Briefly, skin specimens were first washed in Salsol A solution (Human Rt) supplemented with 2% antibiotic/antimycotic solution (Sigma‐Aldrich). Skin samples were then cut into narrow strips and incubated in Dispase solution (Roche Diagnostics, Mannheim, Germany) overnight at 4°C. The epidermis was subsequently separated from the dermis. Fibroblasts were obtained by incubating the dermis in Digestion Mix solution (Sigma‐Aldrich, Collagenase, Hyaluronidase, and Deoxyribonuclease) at 37°C for 2 h. Cell suspensions were filtered through a 100 µm nylon mesh (BD Falcon), and cells were pelleted by centrifugation (Megafuge 16; Thermo Fisher Scientific). Fibroblasts were grown in low glucose DMEM/F12 medium containing 15% FCS, 1% antibiotic/antimycotic (PAA, Pasching, Austria) and 1% l‐glutamine solution (PAA). Fibroblasts were cultured at 37°C and 5% CO2 in humidified conditions. Depending on the cell growth, the medium was changed every 2–4 days, and cells were passaged at 80% of confluence.