THP-1 phagocytosis assay was performed as described before(48 (link)). In brief, biotinylated antigens were coupled to FluoSphere NeutrAvidin beads (Thermo Fisher) and incubated with 10 μl 1:100 diluted plasma for 2h at 37°C to form immune complexes. THP-1 monocytes were added to the beads, incubated for 16 h at 37°C, fixed with 4% para-formaldehyde and analyzed on a iQue analyzer (Intellicyt).
Protocol detail
Phagocytosis of Antigen-Immune Complexes by THP-1 Monocytes
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Massachusetts General Hospital
Other organizations : Children's Clinical University Hospital, University College London
Top 5 similar protocols
Variable analysis
independent variables
- Biotinylated antigens coupled to FluoSphere NeutrAvidin beads
- Phagocytosis of immune complexes by THP-1 monocytes
- Incubation time (2h at 37°C) for forming immune complexes
- Incubation time (16h at 37°C) for phagocytosis by THP-1 monocytes
- Concentration of plasma (1:100 dilution)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!