Integrin-mediated Cell Adhesion Assays

TIMP1, TIMP2, and batimastat were from Sigma-Aldrich. Rabbit anti-MMP14 (hinge region) and mouse anti–integrin β1 antibodies were from Abcam (Cambridge, MA). Mouse anti-cortactin antibody was from Millipore (Billerica, MA). Rabbit anti–p-c-Src (Tyr-419) antibody and Src family kinase inhibitors Dasatinib and SU6656 were from Santa Cruz Biotechnology (Santa Cruz, CA). DQ collagen, Oregon Green 488–conjugated gelatin, mouse anti–cadherin-11, mouse anti–α-catenin, and mouse anti–β-catenin antibodies, Alexa Fluor–conjugated anti-mouse, anti-rat, and anti-rabbit secondary antibodies, and Alexa Fluor–conjugated phalloidin were from Invitrogen. Mouse anti–p120-catenin antibody was from BD Biosciences (San Jose, CA). Mouse anti–cadherin-11 (3H10) was previously raised in this laboratory (Valencia et al., 2004 (link)). DRAQ5 was from Cell Signaling (Danvers, MA). Human phosphokinase antibody array was from R&D Systems (Minneapolis, MN).

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Gu Z., Liu F., Tonkova E.A., Lee S.Y., Tschumperlin D.J, & Brenner M.B. (2014). Soft matrix is a natural stimulator for cellular invasiveness. Molecular Biology of the Cell, 25(4), 457-469.

Publication 2014

TIMP1 TIMP2 batimastat Dasatinib SU6656 DQ collagen Oregon Green 488–conjugated gelatin Alexa Fluor–conjugated phalloidin DRAQ5 Human phosphokinase antibody array

Anti antibodies Anti antibody Antibody Batimastat Cadherin 11 Catenin Collagen Cortactin Dasatinib Gelatin Human Inhibitors Integrin Mmp14 Mouse Oregon green 488 P120 catenin Phalloidin Phosphokinase Rabbit Src family kinase Su6656 Timp1 Timp2 β catenin

Corresponding Organization :

Other organizations : The University of Texas MD Anderson Cancer Center, Brigham and Women's Hospital, Harvard University, Mayo Clinic in Florida

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Variable analysis

independent variables

TIMP1
TIMP2
Batimastat
Rabbit anti-MMP14 (hinge region) antibody
Mouse anti–integrin β1 antibody
Mouse anti-cortactin antibody
Rabbit anti–p-c-Src (Tyr-419) antibody
Src family kinase inhibitors Dasatinib and SU6656

dependent variables

DQ collagen
Oregon Green 488–conjugated gelatin
Mouse anti–cadherin-11
Mouse anti–α-catenin
Mouse anti–β-catenin
Mouse anti–p120-catenin
Alexa Fluor–conjugated phalloidin

control variables

Alexa Fluor–conjugated anti-mouse, anti-rat, and anti-rabbit secondary antibodies
DRAQ5

positive controls

Mouse anti–cadherin-11 (3H10) previously raised in this laboratory (Valencia et al., 2004)

negative controls

None specified

Annotations

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