Hydrophis curtus venom was a pooled sample from 10 adult snakes collected in the waters of Penang Island (Malaysia). The antivenom tested was Sea Snake Antivenom (SSAV) produced by CSL Ltd. (Melbourne, Australia, Seqirus Ltd. currently). The antivenom was in (liquid form) contained F(ab’)2 from horses hyperimmunized against the venoms of Hydrophis schistosus (beaked sea snake, obtained from the waters of Penang Island, Malaysia) and the Australian Notechis scutatus (common tiger snake). The protein concentration of the antivenom was 217.2 ± 3.0 mg/mL, determined in a previous study from the laboratory [25 (link)].
The chemicals and reagents used were primarily supplied by Sigma-Aldrich (Saint Louis, MO, USA) and were of analytical grade. The molecular weight marker Spectra™ Multicolor Broad Range Protein Ladder (10–260 kDa), trypsin (mass spectrometry grade), HPLC grade acetonitrile (ACN) and trifluoroacetic acid (TFA) were purchased from Thermo Scientific™ Pierce™ (Waltham, MA, USA). Millipore ZipTip® C18 Pipette Tips and LiChrospher® WP 300 RP-18 (5 µm) were supplied by Merck (Burlington, MA, USA).
The chemicals and reagents used were primarily supplied by Sigma-Aldrich (Saint Louis, MO, USA) and were of analytical grade. The molecular weight marker Spectra™ Multicolor Broad Range Protein Ladder (10–260 kDa), trypsin (mass spectrometry grade), HPLC grade acetonitrile (ACN) and trifluoroacetic acid (TFA) were purchased from Thermo Scientific™ Pierce™ (Waltham, MA, USA). Millipore ZipTip® C18 Pipette Tips and LiChrospher® WP 300 RP-18 (5 µm) were supplied by Merck (Burlington, MA, USA).
Free full text: Click here
