Viral Nucleic Acid Extraction using NucliSens Kit

The viral nucleic acids were extracted from the PEG pellets and the ultrafiltered concentrate using the NucliSens extraction kit (BioMerieux, France) on a Kingfisher 96 Flex system (Thermo Scientific, USA) as described previously [32 (link), 34 (link)]. The final volume of the eluent was 100 μl. To assess extraction performance and cross-contamination, extraction positive (deionised water spiked with phi6) and negative controls (distilled water) were used.

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Farkas K., Williams R., Alex-Sanders N., Grimsley J.M., Pântea I., Wade M.J., Woodhall N, & Jones D.L. (2023). Wastewater-based monitoring of SARS-CoV-2 at UK airports and its potential role in international public health surveillance. PLOS Global Public Health, 3(1), e0001346.

Publication 2023

NucliSens extraction kit Kingfisher 96 Flex system

Nucleic acids Pellets

Corresponding Organization : Murdoch University

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Variable analysis

independent variables

Extraction method using NucliSens extraction kit on a Kingfisher 96 Flex system

dependent variables

Viral nucleic acid yield

control variables

Extraction positive control (deionised water spiked with phi6)
Extraction negative control (distilled water)

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