Immunohistochemical Analysis of Breast Cancer

Immunohistochemistry for vimentin and E-cadherin were done on formalin-fixed, paraffin-embedded tissue of previously described breast cancer samples [13 (link), 16 (link)]. Vimentin was detected using ready-to-use mouse anti-human vimentin antibody from Ventana (Cat# 790-2917) and were stained using automated staining platform (Ventana). Immunohistochemical staining of E-cadherin was done manually using rabbit anti E-cadherin antibody (clone EP00y) overnight at a dilution of 1:500. Envision + polymer (ready to use; Dako) was used as a secondary antibody. Color was developed with 3,3′-diaminobenzidine (DAB) and instant hematoxylin (Shandon) was used for counterstaining.

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Alsuliman A., Colak D., Al-Harazi O., Fitwi H., Tulbah A., Al-Tweigeri T., Al-Alwan M, & Ghebeh H. (2015). Bidirectional crosstalk between PD-L1 expression and epithelial to mesenchymal transition: Significance in claudin-low breast cancer cells. Molecular Cancer, 14, 149.

Publication 2015

automated staining platform Envision + polymer

Anti antibody Antibody Cadherin Cancer of breast Clone Dilution E cadherin Embedded paraffin Formalin Hematoxylin Human Immunohistochemistry Mouse Polymer Rabbit Vimentin

Corresponding Organization :

Other organizations : King Faisal Specialist Hospital & Research Centre, Alfaisal University

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Variable analysis

independent variables

Immunohistochemistry for vimentin and E-cadherin

dependent variables

Expression of vimentin and E-cadherin in breast cancer samples

control variables

Formalin-fixed, paraffin-embedded tissue of previously described breast cancer samples

controls

Positive control: Not specified
Negative control: Not specified

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