We analysed 21 114 complete bacterial and archaeal genomes from NCBI RefSeq (ftp://ftp.ncbi.nlm.nih.gov/genomes/refseq/, last accessed in March 2021) [46 (link)], representing 5840 species of Bacteria and 288 species of Archaea. HMM profiles were extracted from the Panther database (version 15) [47 (link)]. TnSeq data was accessed on the Fitness browser https://fit.genomics.lbl.gov/cgi-bin/myFrontPage.cgi [48 (link)].
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