MTT Assay for Cell Viability Quantification

MTT assay is a colorimetric assay used to quantify the survival of viable cells (38 (link), 39 (link)). In brief, 3 x 10⁴ cells were seeded into 96-well plates overnight. Cell lines were treated with either paclitaxel (PTX) (EbeweR, SANDOZ, Novartis, Basel Switzerland) or cisplatin (CIS) (Accord Healthcare Pty Ltd, Melbourne, Australia) and MTT assay was performed by replacing culture media with 100µL of thiazolyl blue tetrazolium (MTT) solution (Sigma-Aldrich) dissolved in 1x PBS solution (0.5mg/mL final concentration) (Sigma-Aldrich). Cells were incubated for 2 hours at 37°C in 5% CO₂ humidity. Media was discarded and replaced with 100µL of dimethyl sulfoxide (DMSO). Absorbance was read at OD595nm using the CLARIOstar Plate Reader (BMG Labtech, Germany) and data was analysed by MARS Data Analysis Computer Software (BMG Labtech, Mornington, Victoria, Australia).