Biochemical Analysis of Crucian Carp

Live crucian carp (Carassius auratus) were purchased from Tongwei Sanlian Aquatic Products Trading Center (Chengdu, China), with weights of 230 ± 10 g and body lengths of 20 ± 2 cm. For all the experiments, intact, vigorous, and healthy crucian carp were used and cultured in polyethylene tank (0.5 × 0.4 × 0.3 m), with running water (20 ± 1 °C, pH 7.0 ± 0.5, the average dissolved oxygen was 6.0 mg/L), for 48 h before the experiments. According to the decision of the Animal Ethics Committee of Chengdu University, the fish used in experiments were washed with running water, dried with filter paper, and killed by knocking the head. Collect blood samples from the fish tail vein and the fish back muscle for biochemical analysis. Guanosine 5′-monophosphate disodium salt hydrate (5′-GMP-Na2, with purity 99.0%), cytidine 5′-monophosphate (5′-CMP, with purity 99.0%), uridine 5′-monophosphate disodium salt (5′-UMP-Na2, with purity 99.0%), 5′-inosinic acid disodium salt hydrate (5′-IMP-Na2, with purity 99.0%), and adenosine 5′-monophosphate disodium salt (5-AMP-Na2, with purity 99.0%) were purchased from Sigma-Aldrich (St. Louis, MO, USA); potassium dihydrogen phosphate, trichloroacetic acid, hydrochloric acid, and other chemical reagents were purchased from Chengdu Cologne Chemical Co., Ltd. (Chengdu, China); a lactate kit and glucose kit were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).