Antimicrobial Susceptibility Testing Protocol

Both the antibacterials and antifungals were evaluated for their activity, as per the standard protocol of the Clinical and Laboratory Standards Institute, using an overnight grown bacterial/fungal suspension as described earlier [8 (link),9 (link),12 (link),46 (link)]. The minimum inhibitory concentration (MIC) required for inhibiting the growth of the microorganisms was determined for each of the antibacterials (Aminoglycosides: Amikacin, Gentamicin, and Tobramycin; β-lactam: Ampicillin; Cephalosporins: Cefuroxime, Ceftriaxone, Cefepime, and Cefazolin; Fluoroquinolones: Gatifloxacin, Moxifloxacin, Ciprofloxacin and Ofloxacin; Amphenicols: Chloramphenicol; Macrolide: Azithromycin; Nitroimidazole: Metronidazole; Lincosamide: Clindamycin and Lincomycin; Tetracycline: Monocycline) and the antifungal drugs (Amphotericin B, Caspofungin, Fluconazole, Itraconazole, Natamycin and Voriconazole). The susceptibility tests were determined in triplicate.

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Shivaji S., Nagapriya B, & Ranjith K. (2023). Differential Susceptibility of Mixed Polymicrobial Biofilms Involving Ocular Coccoid Bacteria (Staphylococcus aureus and S. epidermidis) and a Filamentous Fungus (Fusarium solani) on Ex Vivo Human Corneas. Microorganisms, 11(2), 413.

Publication 2023

Amikacin Aminoglycosides Amphenicols Amphotericin b Ampicillin Antibacterials Antifungal drugs Azithromycin Bacterial Caspofungin Cefazolin Cefepime Ceftriaxone Cefuroxime Cephalosporins Chloramphenicol Ciprofloxacin Clindamycin Clinical protocol Fluconazole Fluoroquinolones Gatifloxacin Gentamicin Itraconazole Lactam Lincomycin Lincosamide Macrolide Metronidazole Minimum inhibitory concentration Moxifloxacin Natamycin Nitroimidazole Ofloxacin Susceptibility Tetracycline Tobramycin Voriconazole

Corresponding Organization : L V Prasad Eye Institute

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Variable analysis

independent variables

Antibacterials (Aminoglycosides: Amikacin, Gentamicin, and Tobramycin; β-lactam: Ampicillin; Cephalosporins: Cefuroxime, Ceftriaxone, Cefepime, and Cefazolin; Fluoroquinolones: Gatifloxacin, Moxifloxacin, Ciprofloxacin and Ofloxacin; Amphenicols: Chloramphenicol; Macrolide: Azithromycin; Nitroimidazole: Metronidazole; Lincosamide: Clindamycin and Lincomycin; Tetracycline: Monocycline)
Antifungal drugs (Amphotericin B, Caspofungin, Fluconazole, Itraconazole, Natamycin and Voriconazole)

dependent variables

Minimum inhibitory concentration (MIC) required for inhibiting the growth of the microorganisms

control variables

Standard protocol of the Clinical and Laboratory Standards Institute
Overnight grown bacterial/fungal suspension

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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