Immunoblotting Analysis of Peroxisomal Proteins

Polyclonal rabbit antibodies were raised against Pex13 [31 (link)] and Por1 [32 (link)]. Monoclonal mouse antibodies were raised against GFP (Sigma-Aldrich/Merck, Germany) and Pgk1 (Invitrogen, Karlsruhe, Germany). Immuno-reactive complexes were visualized using the IRDye 800CW goat anti-rabbit IgG or IRDye 680RD goat anti-rabbit secondary antibody (Li-COR Bioscience, Bad Homburg, Germany) followed by detection using the “Infrarot Imaging System“ (Li-COR Bioscience, Bad Homburg, Germany). The intensity of free anti-Pex13 signals on the Western blots was calculated by Image Studio Lite, LI-COR Bioscience.

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Mastalski T., Brinkmeier R, & Platta H.W. (2020). The Peroxisomal PTS1-Import Defect of PEX1- Deficient Cells Is Independent of Pexophagy in Saccharomyces cerevisiae. International Journal of Molecular Sciences, 21(3), 867.

Publication 2020

IRDye 800CW goat anti-rabbit IgG IRDye 680RD goat anti-rabbit secondary antibody Infrarot Imaging System Image Studio Lite

Anti antibody Anti igg Antibodies Goat Irdye 800cw Monoclonal antibodies Mouse Rabbit Western blots

Corresponding Organization : Ruhr University Bochum

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Variable analysis

independent variables

Polyclonal rabbit antibodies raised against Pex13
Polyclonal rabbit antibodies raised against Por1
Monoclonal mouse antibodies raised against GFP
Monoclonal mouse antibodies raised against Pgk1

dependent variables

Intensity of free anti-Pex13 signals on the Western blots

control variables

IRDye 800CW goat anti-rabbit IgG or IRDye 680RD goat anti-rabbit secondary antibody
Infrarot Imaging System (Li-COR Bioscience, Bad Homburg, Germany)

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