IHC was performed on selected sections, as described [12 (link), 15 (link)]. The following primary antibodies were used: histone H3-K27M (Millipore/Sigma, Burlington, MA), IDH1-R132H (DIA-H09, Dianova, Hamburg, Germany), p53 (DO-7), vimentin (V9), Ki-67 (30-9) (Roche/Ventana Medical Systems Inc., Tucson, AZ), Olig-2 (387M-15), GFAP (EP672Y) (Ventana/ Cell Marque, Rocklin, CA).
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