The particle size (PS), polydispersity index (PDI), and zeta potential (ZP) of the developed canagliflozin-loaded nanomicelles were determined by using a Malvern Panalytical Ltd Zetasizer. Briefly, 40 mg of CFZ-SNMSD was dispersed in 20 ml deionized water, stirring at 300 rpm for 1 hour using a magnetic stirrer (Witeg labortechnik GMBH, Germany), filtering by 0.45 µm syringe filter and analyzed [13] .
The particle size stability of the CFZ-SNMSD formula to an eventual dilution with the gastrointestinal tract (GIT) fluid, a process that usually occurs after oral administration, was estimated by analyzing the PS and PSI of the CFZ-SNMSD dispersion diluted with water in a ratio of 1:20 [14] Transmission electron microscopy (TEM)
The nanomicelles' size and morphology were studied by transmission electron microscopy (TEM), using Tecnai 12, TEM apparatus, Philips Company, Holland, where a drop of the solution was applied to a copper grid, followed by phosphotungstic acid and deionized water, dried and mounted on a grid holder. Their morphology was studied at an acceleration voltage of 120 kV [15] (link).
The particle size stability of the CFZ-SNMSD formula to an eventual dilution with the gastrointestinal tract (GIT) fluid, a process that usually occurs after oral administration, was estimated by analyzing the PS and PSI of the CFZ-SNMSD dispersion diluted with water in a ratio of 1:20 [14] Transmission electron microscopy (TEM)
The nanomicelles' size and morphology were studied by transmission electron microscopy (TEM), using Tecnai 12, TEM apparatus, Philips Company, Holland, where a drop of the solution was applied to a copper grid, followed by phosphotungstic acid and deionized water, dried and mounted on a grid holder. Their morphology was studied at an acceleration voltage of 120 kV [15] (link).
