Glucose-Stimulated Insulin Secretion Assay

After preincubation in modified Krebs-Ringer for 1 h, cells were sequentially stimulated with low (0 mmol/L) and high glucose (20 mmol/L) for 1 h (each stimulation) as previously described (34 (link)). Insulin secreted and insulin content from lysed cells were measured using a human insulin ELISA kit (Mercodia, Uppsala, Sweden) following the manufacturer’s instructions. The amount of secreted insulin as % of total insulin was calculated as previously described (35 (link)) and data were normalized to insulin secretion at 20 mmol/L glucose in vehicle-treated cells without IFNα (considered as 100%). See Supplementary Material for further details.