Infection of mice with Listeria monocytogenes and subsequent analysis of T cell effector function were performed as previously described [27] (link), except that a cocktail of IL-23 (50 ng/ml; BioLegend), IL-1 (10 ng/ml; BioLegend), and Pam3Cys (1 μg/ml; Invivogen) was used to elicit IL-17 production from both γδ-17 and DN-17 cells.
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