Immunohistochemical Biomarker Evaluation

Immunohistochemistry was performed on paraffin-embedded tissues sections (3-4 μm-thick) using the following primary antibodies: ER-α (clone 6F11, monoclonal; Novocastra, Newcastle-Upon-Tyne, UK), PgR (clone PgR636, monoclonal; DakoCytomation, Glostrup, Denmark) and HER2 (A0485, polyclonal; DakoCytomation). Diaminobenzidine (Dako) was used as the chromogen. ER and PgR expression was considered positive when at least 10% of invasive tumoral cells exhibited nuclear staining, regardless of intensity. For HER2, the immunohistochemical score was assigned according to the Herceptest scoring system [19 (link)].

Free full text: Click here

Youssef S.S., Mohammad M.M, & Ezz-El-Arab L.R. (2015). Clinical Significance of Serum IL-12 Level in Patients with Early Breast Carcinoma and Its Correlation with Other Tumor Markers. Open Access Macedonian Journal of Medical Sciences, 3(4), 640-644.

Publication 2015

ER-α (clone 6F11, PgR (clone PgR636, Diaminobenzidine

Antibodies Cells Chromogen Clone Embedded paraffin Her2 Immunohistochemistry Tissues Tumoral

Corresponding Organization :

Other organizations : Ain Shams University

Top 5 similar protocols

Variable analysis

independent variables

Primary antibody used: ER-α (clone 6F11, monoclonal; Novocastra, Newcastle-Upon-Tyne, UK), PgR (clone PgR636, monoclonal; DakoCytomation, Glostrup, Denmark) and HER2 (A0485, polyclonal; DakoCytomation)

dependent variables

ER and PgR expression (considered positive when at least 10% of invasive tumoral cells exhibited nuclear staining, regardless of intensity)
HER2 immunohistochemical score (assigned according to the Herceptest scoring system)

control variables

Paraffin-embedded tissue sections (3-4 μm-thick)
Diaminobenzidine (Dako) used as the chromogen

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!