Cell suspensions were washed in PBS, pelleted, incubated in RIPA lysis buffer, and immunoblotting was performed as described previously28 (link). The following antibodies were utilized: α-FAK C-20 (Santa Cruz, Dallas, TX; [0.2 μg/ml]), α-ERK p44/42 (Cell Signaling, Danvers, MA; [1:2000]), and α-AKT (Cell Signaling; [1:1000]).
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