DMBA/TPA-Induced Mouse Skin Histopathology

Example 10

H&E staining was performed to examine the histopathology of mouse skin tissues (FIG. 11). The typical skin architecture with epidermis, dermis, subcutis, muscle and hair follicles were observed in sham group mice. Topical application of DMBA/TPA resulted in an increase in epidermal thickness which is suggested to be abnormal proliferation and hyperplasia of the epidermis. The irregular thickness of the epidermis was attenuated by KWM-EO, LM-EO and L+C combination treatment. In DMBA-initiated and TPA-promoted skin, intraperitoneal injection of PLX4032 exacerbated the proliferation and hyperplasia of the epidermis. Mint EOs and major compound application also notably inhibited the unnatural thickness of the epidermis.