Evaluating Microbial Load Quantification

Different samples representing biotechnology, agriculture, medicine, food microbiology, environmental microbiology and applied microbiology where there was no clear idea about the prevalent bacterial or yeast cfu in the sample were tested through the SP-SDS approach. The preferred dilutions from the anchored stocks included 10⁰–10⁵ or 10¹–10⁶ for liquid samples, and 10¹–10⁶ for solid samples avoiding the particulate 10⁰. Further, SP-SDS was tried for parallel testing of two or multiple samples in a plate. This included testing the effect due to different diluents on E. cloacae where the 10⁰ stock in FDW was taken through serial dilution in saline (NaCl 9 g l⁻¹), phosphate buffered saline (PBS), peptone–water (10 g l⁻¹ peptone and 5 g l⁻¹ NaCl; pH 7.2) [1] , peptone-salt (1 g l⁻¹ each peptone and NaCl; pH 7.0; [23] (link)) or nutrient broth (pH 7.4) employing FDW as control. In another trial, E. cloacae dilutions prepared in FDW and peptone salt was monitored with SP–SDS after static incubation over 5 h at 20 min intervals during the initial one hour and hourly thereafter employing the decimal dilutions 10³–10⁸. Further experimental details are provided under Results and Discussion.