Crude extracts were mixed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) loading buffer, boiled for 5 min, and analyzed by 10% SDS-PAGE. Resolved proteins were transferred onto nitrocellulose membranes, detected by enhanced chemiluminescence according to the manufacturer’s instructions (Amersham, Buckinghamshire, UK), and analyzed using a Molecular Image ChemiDoc XRS system (Bio-Rad; Hercules, CA) (23 (link)). Densitometry was performed using Image J software (NIH; Bethesda, MD). β-Actin was used to confirm equal protein loading for all samples.
Protocol detail
Protein Detection by SDS-PAGE and Western Blot
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Actin
Buffer
Chemiluminescence
Crude extracts
Densitometry
Membranes
Nitrocellulose
Protein
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Corresponding Organization :
Other organizations : University of Ulsan, Ulsan College, Konyang University, Korea Institute of Science and Technology
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- Crude extracts were mixed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) loading buffer
- Resolved proteins were transferred onto nitrocellulose membranes
- Proteins were detected by enhanced chemiluminescence
- Proteins were analyzed using a Molecular Image ChemiDoc XRS system
- β-Actin was used to confirm equal protein loading for all samples
- No positive or negative controls were explicitly mentioned in the input text.
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