Human U937 monocytes (EC85011440) were commercially purchased from the European Collection of Animal Cell Culture, UK. Moreover, we used linagliptin compounds, FBS, LPS, phorbol 12-myristate 13-acetate, and Roswell Park Memorial Institute medium (RPMI 1640), as described in our previous study.7 (link) Sitagliptin was obtained from Santa Cruz Biotechnology, Inc. (Bergheimer, Heidelberg, Germany), and Loxo was obtained from Wako Pure Chemical Industries Ltd. (Osaka, Japan).
Briefly, cells were cultured in the presence of RPMI 1640 containing 10% FBS, as previously described.7 (link) The passage number of U937 in this study ranged from two to five. Cells were evaluated in duplicate, and each experiment was repeated three times.
Briefly, cells were cultured in the presence of RPMI 1640 containing 10% FBS, as previously described.7 (link) The passage number of U937 in this study ranged from two to five. Cells were evaluated in duplicate, and each experiment was repeated three times.
