CD68 Immunohistochemistry Staining Protocol

The immunohistochemistry assay was performed according to Yin et al. (26 (link)). The sections were incubated with the primary antibody of CD68 (Ab955, 1: 100, Abcam, Cambridge, UK) at 4 °C overnight and horseradish peroxidase labeled goat anti-mouse IgG antibody (A205719, 1:200, Abcam, Cambridge, UK) at room temperature for 1 h. The color reaction was performed with diaminobenzidine chromogen solution (Dako, Carpinteria, USA). Brown-yellow particles represented the positive expression of CD68 protein and the blue particles represented the nucleus stained by hematoxylin (Sigma, USA).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Li X., Chen Z., Ni Y., Bian C., Huang J., Chen L., Xie X, & Wang J. (2021). Tumor-associated macrophages secret exosomal miR-155 and miR-196a-5p to promote metastasis of non-small-cell lung cancer. Translational Lung Cancer Research, 10(3), 1338-1354.

Publication 2021

Ab955 A205719 diaminobenzidine chromogen solution hematoxylin

Anti igg Antibody Assay Chromogen Goat Horseradish peroxidase Immunohistochemistry Mouse Nucleus Protein

Corresponding Organization : Southeast University

Other organizations : Huaian First People’s Hospital

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

Primary antibody of CD68 (Ab955, 1: 100, Abcam, Cambridge, UK)

dependent variables

Positive expression of CD68 protein (represented by brown-yellow particles)
Nucleus stained by hematoxylin (represented by blue particles)

control variables

Incubation with primary antibody at 4 °C overnight
Incubation with horseradish peroxidase labeled goat anti-mouse IgG antibody (A205719, 1:200, Abcam, Cambridge, UK) at room temperature for 1 h
Color reaction performed with diaminobenzidine chromogen solution (Dako, Carpinteria, USA)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!